Coverage statistics for sequence census methods

April, 2010
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Steven N. Evans, Valerie Hower and Lior Pachter

We study the shape of coverage functions resulting from the sequencing of random genome fragments, and show that they can be described by Galton-Watson trees. This extends standard analyses of shotgun sequencing that focus on coverage statistics at individual sites, and provides a null model for detecting deviations from random coverage in high-throughput sequence census based experiments such as ChIP-Seq.

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